Adenovirus type 2 DNA replicated in arginine-starved KB cells is infectious.
نویسندگان
چکیده
Adenovirus type 2 DNA from extracts of KB cells rendered nonpermissive by arginine starvation is infectious.
منابع مشابه
Synthesis of virus deoxyribonucleic acid during abortive infection of simian cells by human adenoviruses.
Rapp, Fred (Baylor University College of Medicine, Houston, Tex.), Lawrence A. Feldman, and Manley Mandel. Synthesis of virus deoxyribonucleic acid during abortive infection of simian cells by human adenoviruses. J. Bacteriol. 92:931-936. 1966.-Inoculation of green monkey kidney cells (GMK) with adenovirus types 2 or 12, under conditions where neither infectious virus was synthesized, resulted ...
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When exponentially growing KB cells were deprived of arginine, cell multiplication ceased after 12 h but viability was maintained throughout the experimental period (42-48 h). Although tritiated thymidine ([(3)H]TdR) incorporation into acid-insoluble material declined to 5 percent of the initial rate, the fraction of cells engaged in DNA synthesis, determined by autoradiography, remained consta...
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DNA synthesis in cells deprived of arginine was examined. Three lines of evidence indicated that tritiated thymidine ([3H]TdR) incorporation in arginine-starved cells was due to replicative rather than repair DNA synthesis. (a) When made in the presence of bromodeoxyuridine, the [3H]TdR-labeled DNA sedimented at hybrid density in isopycnic gradients. (b) As determined by the diphenylamine react...
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ورودعنوان ژورنال:
- Journal of virology
دوره 44 1 شماره
صفحات -
تاریخ انتشار 1982